Characterization of plasma-derived extracellular vesicle proteomics for biomarker development of PSP and CBD

Pipeline Grant

Principal Investigator: Dr. Yang You, Mayo Clinic Jacksonville

Our research aims to develop new blood-based biomarkers that can facilitate the diagnosis of progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD). Currently, there are no reliable blood tests to distinguish either PSP or CBD from non-demented cases and other neurodegenerative diseases such as Alzheimer’s disease (AD). Our major goal is to find a way to diagnose PSP and CBD early, giving patients a better chance at treatment. We’re focusing on something called “extracellular vesicles” (EVs), tiny packages that cells release into the bloodstream. These EVs carry important molecules which are dynamically changed when diseases are progressing. By studying the EVs in blood, we hope to discover unique markers for PSP and CBD. At first, we’re improving the way we separate EVs from blood samples. We want to get rid of the other stuffs we don’t need in the blood. We’ll use a special tool and make sure it works by testing different methods. Next, we’ll study the EVs isolated from people with PSP, CBD, AD, and those without any brain issues. We’re looking for EVs that have special cargo proteins which may be linked to these diseases. We’ll use high-tech methods to sort through all the information and identify the most promising proteins. Last, we’ll confirm our findings using powerful microscopes and advanced equipment. If our answers are correct, we’ll have an easy way to help diagnose PSP or CBD by simply using the blood. Our work is essential because early diagnosis of these neurodegenerative diseases can lead to better treatments and improved lives for those affected. Our research could have a chance to bring us closer to this goal.